Laboratory of Analytical Chemistry

Laboratory of Analytical Chemistry is a service group providing an essential analytical support for the research groups of our Institute. Our laboratories are well equipped with modern analytical instruments for the analysis of inorganic or organic compounds. Our work is mainly focused on structure elucidation, identification and quantification of organic compounds and their mixtures both synthetic or isolated from natural matrices. We design and develop suitable analytical method for each client requirement. For organic compounds analysis we are using HPLC-HRMS or HPLC-DAD methods, GC-FID or GC-MS methods and NMR spectroscopy. We also perform metal elements analysis (including heavy metals, rare earth metals) in a variety of sample matrices (MP-AES, ICP-OES), liquid or solid samples after digestion. In addition to all above mentioned techniques we are able to provide analysis using classical titration methods.

Instrumentation

• LC Systems
• NMR Spectroscopy
• GC Systems
• Elemental Analysis
• Other instruments

LC Systems

HPLC-HRMS/MS – The liquid chromatography system Dionex Ultimate3000 (ThermoScientific) coupled to tandem mass spectrometer micrOTOF-Q III (Bruker Daltonik) equipped with Quadrupole-Time-of-Flight analyser (Q-TOF). Utilization of both electrospray ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI) with direct probe option is possible.

HPLC-DAD/ELSD – The liquid chromatography system Dionex Ultimate3000 connected to a Diode Array Detector (DAD) (ThermoScientific) or Evaporative Light Scattering detector (ELSD) (Polymer Laboratories).

HPLC-UV/VIS – Isocratic preparative liquid chromatography system Streamline™ equipped with UV/VIS detector (WATREX).

NMR Spectroscopy

Bruker Avance III HD 400 MHz NMR spectrometer (Bruker) equipped with 5mm probe with inner coil for heteronuclear measurements (from ¹⁵N to ³¹P + ¹⁹F).

Responsible and contact person: Ing. Lucie Červenková Šťastná, Ph.D. Stastna@icpf.cas.cz

Varian Unity Inova 500 MHz NMR spectrometer (Varian) equipped with 5mm probe with inner coil for ¹H measurements.

Responsible and contact person: Ing. Štěpán Horník, Ph.D. hornik@icpf.cas.cz

GC Systems

GC-MS/FID – Gas chromatography system Trace 1310 coupled to ISQ 7000 mass spectrometer (single quadrupole analyser) or flame ionization detector (FID) (ThermoScientific).

GC-FID – Gas chromatography system Scion 456 coupled to flame ionization detector (FID) (Bruker).

Elemental analysis

MP-AES Agilent 4200, Microwave Plasma Atomic Emission Spectroscopy (Agilent)

ICP-OES AVIO 500, Inductively Coupled Plasma Optical Emission Spectroscopy (PerkinElmer)

Responsible and contact person: Ing. Ondřej Táborský taborsky@icpf.cas.cz

Other instruments

Evolution 220 UV-VIS spectrophotometer (ThermoScientific)