Characterization and purification of carbosilane dendritic compounds

The synthesis of dendritic molecules is inherently connected with the development of innovative purification and characterization methods. We are interested in detailed analysis of dendritic structure and we were able to elucidate the structure and origin of most defects accompanying the synthesis of carbosilane dendrimers as well as their peripheral functionalization. Especially in soft ionization mass spectrometry (ESI, MALDI), the high symmetry and repetitive structure of dendrimers have an amplification effect and lead to higher sensitivity of the method to defects. This information is important for further optimization of synthetic protocols in order to obtain high-purity products suitable for bioorganic applications. Implementation of nanofiltration techniques, both in the aqueous phase and in organic solvents, enabled us to further increase the purity of our products and also to recycle valuable reagents that have to be usually applied in excess.

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